Liberate your approach with our comprehensive range of conjugation solutions. Featuring the 30 most popular labels, our chart will allow you to quickly choose what fluorochromes are the most suitable for your next multiplex experiment. Our comprehensive fluorochrome chart will guide you through each of the steps involved in selecting a fluorochrome. In this case, you must consider the spectral overlap between fluorochromes and other factors to minimize potential problems such as fluorescence spillover. The task becomes even more complicated when designing a multiplex panel that requires two or more fluorochromes. Selecting the right fluorochrome may seem difficult if you do not have adequate tools.

Choosing the right fluorochromes for a multiplex panel Therefore, the fluorochrome brightness will depend on its ability to absorb light and the efficiency at which the absorbed light is converted into emitted light. Fluorochromes differ in the intensity at which they emit light. You can find commercially available fluorescent dyes that absorb and emit light at specific wavelengths across the whole visible spectrum, including the infrared region. Many different fluorochromes have been developed, each one with a particular emission and excitation fluorescence spectra. This fluorescence is the read-out signal provided by the instrument. They also provide project support services through the Project Office.

To this end, Company ABC provides senior and intermediate management staff.

The light emitted by the fluorochrome is then filtered so that each sensor will detect fluorescence only within the filter's range. The Company ABC team has overall responsibility for coordinating the day-to-day efforts of the XYZ Partners. Instruments requiring the use of fluorescent dyes, such as fluorescence microscopes and flow cytomeres, are equipped with lasers producing light at a particular wavelength so they can excite fluorochromes capable of absorbing light at that specific wavelength. The wavelengths at which the fluorochrome absorbs and emits light are known as the fluorochrome's excitation and emission spectra, respectively (or just fluorescence spectra). Fluorochromes absorb light energy of a specific wavelength and re-emit it at a longer wavelength. ViewВ AlexaFluor В®В secondary antibodiesВ or find out more about our conjugated primary antibodies and other conjugation solutions.ДЇluorescent dyes (or fluorochromes) are commonly used as detection reagents in various applications such as cellular imaging and flow cytometry.

Abc flowchart viewer